Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum.

Phospholipid-protein interactions in the Ca2+-adenosine triphosphatase of sarcoplasmic reticulum.

Ca2+-adenosine triphosphatase from sarcoplasmic reticulum has been delipidated by gel filtration through a Sephadex G-200 column equilibrated with buffer containing cholate. The delipidated Ca2+-adenosine triphosphatase had negligible adenosine triphosphatase activity, but up to 50% of the ATPase activity was restored when the delipidated enzyme was recombined with phosphilipids. It was shown w...

Cytochemical Studies of Adenosine Triphosphatase Activity in the Sarcoplasmic Reticulum

In an earlier study (Padykula and Gauthier, 1963) two distinct ATPases 1 (mitochondrial and myofibrillar ATPase) were localized within skeletal muscle fibers of the rat diaphragm, using the light microscope. A third ATPase active at pH 7.2 and dependent on sulfhydryl groups appeared to have its origin in the sarcoplasmic reticulum. In transverse sections of the muscle fibers, the final reaction...

A borohydride reduction method for characterization of the acyl phosphate linkage in proteins and its application to sarcoplasmic reticulum adenosine triphosphatase.

A new method for identification and characterization of an acyl phosphate linkage in phosphorylated proteins is presented. The method involves reductive cleavage of the acyl phosphate bond with sodium [3H]borohydride to form a labeled aminohydroxy acid residue. [3H]Borohydride reduction of the phosphorylated (Ca2+, Mg2+)-adenosine triphosphatase of sarcoplasmic reticulum, followed by analysis o...

The calcium binding sites involved in the regulation of the purified adenosine triphosphatase of the sarcoplasmic reticulum.

Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle.

Antibody was raised in chickens against purified sarcoplasmic-reticulum Ca2+-activated ATPase (Ca2+-ATPase). The immunological relationship between the Ca2+-ATPase of fast-muscle and slow-muscle sarcoplasmic reticulum was investigated by a one-step and a two-step competitive enzyme-linked immunosorbent assay (ELISA). The results show marked antigenic differences between the membrane-bound Ca2+-...